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Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.

Anti-PGLYRP2 Antibody

HPA043568
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPGLYRP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PGLYRP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114770
  • Target name
    PGLYRP2
  • Target description
    peptidoglycan recognition protein 2
  • Target synonyms
    HMFT0141; N-acetylmuramoyl-L-alanine amidase; peptidoglycan recognition protein L; peptidoglycan recognition protein long; PGLYRPL; PGRPL; PGRP-L; tagL; tagL-alpha; tagl-beta; TAGL-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PD5
  • Protein Name
    N-acetylmuramoyl-L-alanine amidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PGLYRP2 using anti-PGLYRP2 antibody (A09568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGLYRP2 antigen affinity purified polyclonal antibody (Catalog # A09568) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGLYRP2 at approximately 74 kDa. The expected band size for PGLYRP2 is at 62 kDa.
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