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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA846684LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA846684LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA846684LA01HU at dilution of 1:100

PGLYRP2 Antibody

CSB-PA846684LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPGLYRP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PGLYRP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114770
  • Target name
    PGLYRP2
  • Target description
    peptidoglycan recognition protein 2
  • Target synonyms
    HMFT0141; N-acetylmuramoyl-L-alanine amidase; peptidoglycan recognition protein L; peptidoglycan recognition protein long; PGLYRPL; PGRPL; PGRP-L; tagL; tagL-alpha; tagl-beta; TAGL-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PD5
  • Protein Name
    N-acetylmuramoyl-L-alanine amidase
  • Scientific Description
    May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PGLYRP2 using anti-PGLYRP2 antibody (A09568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGLYRP2 antigen affinity purified polyclonal antibody (Catalog # A09568) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGLYRP2 at approximately 74 kDa. The expected band size for PGLYRP2 is at 62 kDa.
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