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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.

Anti-PHACTR1 Antibody

HPA029757
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPHACTR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PHACTR1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID221692
  • Target name
    PHACTR1
  • Target description
    phosphatase and actin regulator 1
  • Target synonyms
    DEE70, EIEE70, RPEL, RPEL1, dJ257A7.2, phosphatase and actin regulator 1, RPEL repeat containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0D0
  • Protein Name
    Phosphatase and actin regulator 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PHACTR1 using anti-PHACTR1 antibody (A06344-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHACTR1 antigen affinity purified polyclonal antibody (Catalog # A06344-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHACTR1 at approximately 69 kDa. The expected band size for PHACTR1 is at 66 kDa.
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