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Western Blot Positive WB detected in: Hela whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: PHACTR1 antibody at 6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67, 55 kDa Observed band size: 67 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: PHACTR1 antibody at 6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67, 55 kDa Observed band size: 67 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: PHACTR1 antibody at 6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67, 55 kDa Observed band size: 67 kDa

PHACTR1 Antibody

CSB-PA017880LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPHACTR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHACTR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID221692
  • Target name
    PHACTR1
  • Target description
    phosphatase and actin regulator 1
  • Target synonyms
    DEE70, EIEE70, RPEL, RPEL1, dJ257A7.2, phosphatase and actin regulator 1, RPEL repeat containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0D0
  • Protein Name
    Phosphatase and actin regulator 1
  • Scientific Description
    Binds actin monomers (G actin) and plays a role in the reorganization of the actin cytoskeleton and in formation of actin stress fibers. Plays a role in cell motility. Plays a role in the formation of tubules by endothelial cells. Regulates PPP1CA activity. Required for normal cell survival.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHACTR1 using anti-PHACTR1 antibody (A06344-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHACTR1 antigen affinity purified polyclonal antibody (Catalog # A06344-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHACTR1 at approximately 69 kDa. The expected band size for PHACTR1 is at 66 kDa.
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