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Immunohistochemical staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in islet cells.

Anti-PHC1 Antibody

HPA006973
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetPHC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PHC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1911
  • Target name
    PHC1
  • Target description
    polyhomeotic homolog 1
  • Target synonyms
    early development regulator 1 (homolog of polyhomeotic 1); early development regulatory protein 1; EDR1; HPH1; MCPH11; polyhomeotic-like 1; polyhomeotic-like protein 1; RAE28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78364
  • Protein Name
    Polyhomeotic-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHC1 using anti-PHC1 antibody (A06437-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHC1 antigen affinity purified polyclonal antibody (Catalog # A06437-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHC1 at approximately 140 kDa. The expected band size for PHC1 is at 106,130 kDa.
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