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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017891HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017891HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017891HA01HU at dilution of 1:100

PHC1 Antibody

CSB-PA017891HA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPHC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1911
  • Target name
    PHC1
  • Target description
    polyhomeotic homolog 1
  • Target synonyms
    early development regulator 1 (homolog of polyhomeotic 1); early development regulatory protein 1; EDR1; HPH1; MCPH11; polyhomeotic-like 1; polyhomeotic-like protein 1; RAE28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78364
  • Protein Name
    Polyhomeotic-like protein 1
  • Scientific Description
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119, rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHC1 using anti-PHC1 antibody (A06437-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHC1 antigen affinity purified polyclonal antibody (Catalog # A06437-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHC1 at approximately 140 kDa. The expected band size for PHC1 is at 106,130 kDa.
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