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Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Anti-PHF10 Antibody

HPA055649
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPHF10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PHF10
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55274
  • Target name
    PHF10
  • Target description
    PHD finger protein 10
  • Target synonyms
    BAF45A; BRG1-associated factor 45a; BRG1-associated factor, 45-KD, A; PHD finger protein 10; PHD zinc finger protein XAP135; SMARCG4; XAP135
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WUB8
  • Protein Name
    PHD finger protein 10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF10 using anti-PHF10 antibody (A08195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF10 antigen affinity purified polyclonal antibody (Catalog # A08195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF10 at approximately 56 kDa. The expected band size for PHF10 is at 56 kDa.
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Western blot All lanes: PHF10 antibody at 8microg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 3: A2780 whole cell lysate Lane 4: U251 whole cell lysate Lane 5: A549 whole cell lysate Lane 6: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 56, 52 kDa Observed band size: 57 kDa
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