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PHF10 Antibody (HRP)

ABX304813
Abbexa
ApplicationsELISA
Product group Antibodies
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Overview

  • Supplier
    Abbexa
  • Product Name
    PHF10 Antibody (HRP)
  • Delivery Days Customer
    12
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Host
    Rabbit
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF10 using anti-PHF10 antibody (A08195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF10 antigen affinity purified polyclonal antibody (Catalog # A08195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF10 at approximately 56 kDa. The expected band size for PHF10 is at 56 kDa.
Product group Antibodies
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Anti-PHF10 Antibody Picoband(r)A08195-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPHF10
  • SizePrice
Product group Antibodies
References
GeneTex
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Western blot All lanes: PHF10 antibody at 8microg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 3: A2780 whole cell lysate Lane 4: U251 whole cell lysate Lane 5: A549 whole cell lysate Lane 6: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 56, 52 kDa Observed band size: 57 kDa
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