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ProductsBack/Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251
Figure 1. Western Blotting result Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000 dilution.
Figure 1. Western Blotting result Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000 dilution.
Figure 1. Western Blotting result Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000 dilution.

Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251

P00024-6
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAKT1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM251
  • Concentration
    0.5-1 mg/ml
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    Boster Bio Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251 (Catalog # P00024-6). Tested in IHC, WB applications. This antibody reacts with Human.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Yang J, Deng P, Qi Y, et al. MicroRNA-185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator-activated receptor β. Exp Ther Med. 2021,21(4):366. doi: 10.3892/etm.2021.9797
    Read this paper
  • Chen H, Sheng H, Zhao Y, et al. Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway. Med Sci Monit. 2020,26:e928403. doi: 10.12659/MSM.928403
    Read this paper
  • Hu S, Liu YM, Chen-Chen, et al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020,258:118147. doi: 10.1016/j.lfs.2020.118147
    Read this paper
  • Han N, Jiang Y, Gai Y, et al. (11)C-Labeled Pictilisib (GDC-0941) as a Molecular Tracer Targeting Phosphatidylinositol 3-Kinase (PI3K) for Breast Cancer Imaging. Contrast Media Mol Imaging. 2019,2019:1760184. doi: 10.1155/2019/1760184
    Read this paper

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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