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Anti-Phospho-JAK2-Y1007 Antibody

144-50829
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetJAK2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Phospho-JAK2-Y1007 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID3717
  • Target name
    JAK2
  • Target description
    Janus kinase 2
  • Target synonyms
    JTK10, tyrosine-protein kinase JAK2, JAK-2, Janus kinase 2 (a protein tyrosine kinase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60674
  • Protein Name
    Tyrosine-protein kinase JAK2
  • Scientific Description
    Phospho-JAK2-Y1007 pAb
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JAK2 using anti-JAK2 antibody (M00027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK2 antigen affinity purified monoclonal antibody (Catalog # M00027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK2 at approximately 131 kDa. The expected band size for JAK2 is at 131 kDa.
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