Western Blot of mouse heart tissue lysate, using Anti-Phospho-Myl2 (Ser19) rabbit monoclonal antibody (clone RM484) at a 1:1000 dilution. The phospho-specificity of RM484 was verified by peptide blocking using a control non-phosphopeptide or phosphopeptide targeting residue Ser19.
Western Blot of mouse heart tissue lysate, using Anti-Phospho-Myl2 (Ser19) rabbit monoclonal antibody (clone RM484) at a 1:1000 dilution. The phospho-specificity of RM484 was verified by peptide blocking using a control non-phosphopeptide or phosphopeptide targeting residue Ser19.
anti-Phospho-Myl2 (Myosin Light Chain 2v) (Ser19), Rabbit Monoclonal (RM484)
REV-31-1376-00
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMYL2
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-Phospho-Myl2 (Myosin Light Chain 2v) (Ser19), Rabbit Monoclonal (RM484)
- Delivery Days Customer10
- Antibody SpecificityThis antibody reacts to Myl2 (Myosin Light Chain 2v) only when phosphorylated at Ser19. There is no crossreactivity to Myl2 that is not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Myl2 (Ser19).
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM484
- FormulationLiquid
- Gene ID4633
- Target nameMYL2
- Target descriptionmyosin light chain 2
- Target synonymscardiac myosin light chain 2; cardiac ventricular myosin light chain 2; CMH10; MFM12; MLC2; MLC-2; MLC-2s/v; MLC-2v; myosin regulatory light chain 2, ventricular/cardiac muscle isoform; myosin, light chain 2, regulatory, cardiac, slow; myosin, light polypeptide 2, regulatory, cardiac, slow; regulatory light chain of myosin; RLC of myosin; slow cardiac myosin regulatory light chain 2; truncated myosin light chain 2; ventricular myosin light chain 2
- HostRabbit
- IsotypeIgG
- Protein IDP10916
- Protein NameMyosin regulatory light chain 2, ventricular/cardiac muscle isoform
- Scientific DescriptionMyosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber. - Recombinant Antibody. This antibody reacts to Myl2 (Myosin Light Chain 2v) only when phosphorylated at Ser19. There is no crossreactivity to Myl2 that is not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Myl2 (Ser19). Source: Rabbit. Isotype: Rabbit IgG. Immunogen: A phospho-peptide corresponding to human Phospho-Myl2 (Ser19). Applications: WB. Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203