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ProductsBack/Anti-Phospho-PLN-S16 Antibody

Anti-Phospho-PLN-S16 Antibody

144-50826
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLN
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Phospho-PLN-S16 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Gene ID5350
  • Target name
    PLN
  • Target description
    phospholamban
  • Target synonyms
    CMD1P, CMH18, PLB, phospholamban, cardiac phospholamban
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26678
  • Protein Name
    Phospholamban
  • Scientific Description
    Phospho-PLN-S16 pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospholamban/PLN antigen affinity purified polyclonal antibody (Catalog # A01395-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospholamban/PLN at approximately 23 kDa. The expected band size for Phospholamban/PLN is at 6 kDa.
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