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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA018198LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA018198LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA018198LA01HU at dilution of 1:100

PLN Antibody

CSB-PA018198LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLN
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLN Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5350
  • Target name
    PLN
  • Target description
    phospholamban
  • Target synonyms
    cardiac phospholamban; CMD1P; CMH18; PLB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26678
  • Protein Name
    Cardiac phospholamban
  • Scientific Description
    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospholamban/PLN antigen affinity purified polyclonal antibody (Catalog # A01395-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospholamban/PLN at approximately 23 kDa. The expected band size for Phospholamban/PLN is at 6 kDa.
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