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Anti-PI4K2B Antibody

144-65108
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetPI4K2B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PI4K2B Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID55300
  • Target name
    PI4K2B
  • Target description
    phosphatidylinositol 4-kinase type 2 beta
  • Target synonyms
    PI4KIIB, PIK42B, phosphatidylinositol 4-kinase type 2-beta, PI4KII-BETA, phosphatidylinositol 4-kinase type-II beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCG2
  • Protein Name
    Phosphatidylinositol 4-kinase type 2-beta
  • Scientific Description
    PI4K2B pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PI4K2B using anti-PI4K2B antibody (A11023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI4K2B antigen affinity purified polyclonal antibody (Catalog # A11023-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI4K2B at approximately 50 kDa. The expected band size for PI4K2B is at 55,50 kDa.
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