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Immunohistochemical staining of human duodenum shows moderate to strong membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate to strong membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate to strong membranous positivity in glandular cells.

Anti-PI4K2B Antibody

HPA004099
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPI4K2B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PI4K2B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55300
  • Target name
    PI4K2B
  • Target description
    phosphatidylinositol 4-kinase type 2 beta
  • Target synonyms
    PI4KIIB, PIK42B, phosphatidylinositol 4-kinase type 2-beta, PI4KII-BETA, phosphatidylinositol 4-kinase type-II beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCG2
  • Protein Name
    Phosphatidylinositol 4-kinase type 2-beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PI4K2B using anti-PI4K2B antibody (A11023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI4K2B antigen affinity purified polyclonal antibody (Catalog # A11023-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI4K2B at approximately 50 kDa. The expected band size for PI4K2B is at 55,50 kDa.
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