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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of both red and white pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of both red and white pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of both red and white pulp.

Anti-PIGS Antibody

HPA018149
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPIGS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PIGS Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID94005
  • Target name
    PIGS
  • Target description
    phosphatidylinositol glycan anchor biosynthesis class S
  • Target synonyms
    DEE95, GPIBD18, PIG-S, GPI-anchor transamidase component PIGS, GPI transamidase component PIG-S, GPI transamidase subunit, phosphatidylinositol glycan, class S, phosphatidylinositol-glycan biosynthesis class S protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96S52
  • Protein Name
    GPI-anchor transamidase component PIGS
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PIGS using anti-PIGS antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue(HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGS antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGS at approximately 65 kDa. The expected band size for PIGS is at 62,65-70 kDa.
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