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Anti-PIGS Antibody

101-11249
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPIGS
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PIGS Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID94005
  • Target name
    PIGS
  • Target description
    phosphatidylinositol glycan anchor biosynthesis class S
  • Target synonyms
    DEE95, GPIBD18, PIG-S, GPI-anchor transamidase component PIGS, GPI transamidase component PIG-S, GPI transamidase subunit, phosphatidylinositol glycan, class S, phosphatidylinositol-glycan biosynthesis class S protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Human PIGS monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIGS using anti-PIGS antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue(HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGS antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGS at approximately 65 kDa. The expected band size for PIGS is at 62,65-70 kDa.
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