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Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane.

Anti-PIK3CG Antibody

HPA069976
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPIK3CG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PIK3CG Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5294
  • Target name
    PIK3CG
  • Target description
    phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
  • Target synonyms
    IMD97, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K, phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, 1-phosphatidylinositol 3-kinase, PI3-kinase subunit gamma, phosphatidylinositol 3 kinase gamma, p110 gamma, phosphatidylinositol 3-kinase catalytic 110-kD gamma, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, phosphoinositide-3-kinase gamma catalytic subunit, ptdIns-3-kinase subunit gamma, ptdIns-3-kinase subunit p110-gamma, serine/threonine protein kinase PIK3CG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48736
  • Protein Name
    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of PI3K-gamma/PIK3CG using anti-PI3K-gamma/PIK3CG antibody (A01517-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI3K-gamma/PIK3CG antigen affinity purified polyclonal antibody (Catalog # A01517-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI3K-gamma/PIK3CG at approximately 126KD. The expected band size for PI3K-gamma/PIK3CG is at 126KD.
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