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PIK3CG / PI3K Gamma Antibody

LS-C403433
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPIK3CG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PIK3CG / PI3K Gamma Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5294
  • Target name
    PIK3CG
  • Target description
    phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
  • Target synonyms
    IMD97, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K, phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform, 1-phosphatidylinositol 3-kinase, PI3-kinase subunit gamma, phosphatidylinositol 3 kinase gamma, p110 gamma, phosphatidylinositol 3-kinase catalytic 110-kD gamma, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, phosphoinositide-3-kinase gamma catalytic subunit, ptdIns-3-kinase subunit gamma, ptdIns-3-kinase subunit p110-gamma, serine/threonine protein kinase PIK3CG
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PI3K-gamma/PIK3CG using anti-PI3K-gamma/PIK3CG antibody (A01517-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI3K-gamma/PIK3CG antigen affinity purified polyclonal antibody (Catalog # A01517-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI3K-gamma/PIK3CG at approximately 126KD. The expected band size for PI3K-gamma/PIK3CG is at 126KD.
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