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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.

Anti-PIK3R5 Antibody

HPA052247
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPIK3R5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PIK3R5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23533
  • Target name
    PIK3R5
  • Target description
    phosphoinositide-3-kinase regulatory subunit 5
  • Target synonyms
    F730038I15Rik, FOAP-2, P101-PI3K, p101, phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase p101 subunit, phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit, protein FOAP-2, ptdIns-3-kinase p101
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYR1
  • Protein Name
    Phosphoinositide 3-kinase regulatory subunit 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PIK3R5 using anti-PIK3R5 antibody (A02827-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R5 antigen affinity purified polyclonal antibody (Catalog # A02827-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R5 at approximately 97 kDa. The expected band size for PIK3R5 is at 97,55 kDa.
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