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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-PINX1 Antibody

HPA023139
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPINX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PINX1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54984
  • Target name
    PINX1
  • Target description
    PIN2 (TERF1) interacting telomerase inhibitor 1
  • Target synonyms
    Gno1, LPTL, LPTS, Pxr1, PIN2/TERF1-interacting telomerase inhibitor 1, 67-11-3 protein, PIN2-interacting protein 1, TRF1-interacting protein 1, hepatocellular carcinoma-related putative tumor suppressor, liver-related putative tumor suppressor, pin2-interacting protein X1, protein 67-11-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96BK5
  • Protein Name
    PIN2/TERF1-interacting telomerase inhibitor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PINX1 using anti-PINX1 antibody (PB9498). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: HUT Whole Cell Lysate at 40ug, Lane 4: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINX1 antigen affinity purified polyclonal antibody (Catalog # PB9498) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINX1 at approximately 37 kDa. The expected band size for PINX1 is at 37 kDa.
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