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Immunohistochemical staining of human skin shows strong cytoplasmic and membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic and membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic and membranous positivity in squamous epithelial cells.

Anti-PIR Antibody

HPA000697
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetPIR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PIR Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8544
  • Target name
    PIR
  • Target description
    pirin
  • Target synonyms
    pirin, pirin (iron-binding nuclear protein), probable quercetin 2,3-dioxygenase PIR, probable quercetinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00625
  • Protein Name
    Pirin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIR using anti-PIR antibody (A03468-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat RH-35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIR antigen affinity purified polyclonal antibody (Catalog # A03468-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIR at approximately 95 kDa. The expected band size for PIR is at 95 kDa.
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