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Pirin / PIR Antibody

LS-C672320
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPIR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Pirin / PIR Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8544
  • Target name
    PIR
  • Target description
    pirin
  • Target synonyms
    pirin; pirin (iron-binding nuclear protein); probable quercetin 2,3-dioxygenase PIR; probable quercetinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIR using anti-PIR antibody (A03468-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat RH-35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIR antigen affinity purified polyclonal antibody (Catalog # A03468-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIR at approximately 95 kDa. The expected band size for PIR is at 95 kDa.
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