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Anti-PLA2G3 Antibody

144-65087
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetPLA2G3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PLA2G3 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID50487
  • Target name
    PLA2G3
  • Target description
    phospholipase A2 group III
  • Target synonyms
    GIII-SPLA2, SPLA2III, sPLA2-III, group 3 secretory phospholipase A2, group III secreted phospholipase A2, phosphatidylcholine 2-acylhydrolase 3, phosphatidylcholine 2-acylhydrolase GIII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZ20
  • Protein Name
    Group 3 secretory phospholipase A2
  • Scientific Description
    PLA2G3 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLA2G3 using anti-PLA2G3 antibody (A07886-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G3 antigen affinity purified polyclonal antibody (Catalog # A07886-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G3 at approximately 65 kDa. The expected band size for PLA2G3 is at 57 kDa.
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