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PLA2G3 antibody [C1C3]

GTX110780
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPLA2G3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PLA2G3 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID50487
  • Target name
    PLA2G3
  • Target description
    phospholipase A2 group III
  • Target synonyms
    GIII-SPLA2; group 3 secretory phospholipase A2; group III secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 3; phosphatidylcholine 2-acylhydrolase GIII; SPLA2III; sPLA2-III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZ20
  • Protein Name
    Group 3 secretory phospholipase A2
  • Scientific Description
    PLA2G3 belongs to the family of secreted phospholipase A2 (sPLA2; EC 3.1.1.4) proteins. These Ca(2+)-dependent lipolytic enzymes have a conserved Ca(2+)-binding loop and a his-asp dyad in the catalytic site (Murakami et al., 2003 [PubMed 12522102]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLA2G3 using anti-PLA2G3 antibody (A07886-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G3 antigen affinity purified polyclonal antibody (Catalog # A07886-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G3 at approximately 65 kDa. The expected band size for PLA2G3 is at 57 kDa.
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