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Immunohistochemistry analysis in human kidney and pancreas tissues using AMAb90772 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and pancreas tissues using AMAb90772 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and pancreas tissues using AMAb90772 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.

Anti-PLA2R1 Antibody

AMAB90772
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetPLA2R1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLA2R1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0474
  • Conjugate
    Unconjugated
  • Gene ID22925
  • Target name
    PLA2R1
  • Target description
    phospholipase A2 receptor 1
  • Target synonyms
    180 kDa secretory phospholipase A2 receptor; CLEC13C; C-type lectin domain family 13 member C; M-type receptor; phospholipase A2 receptor 1, 180kD; phospholipase A2 receptor 1, 180kDa; PLA2G1R; PLA2IR; PLA2R; PLA2-R; secretory phospholipase A2 receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13018
  • Protein Name
    Secretory phospholipase A2 receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLA2R/PLA2R1 using anti-PLA2R/PLA2R1 antibody (A04442-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2R/PLA2R1 antigen affinity purified polyclonal antibody (Catalog # A04442-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2R/PLA2R1 at approximately 169 kDa. The expected band size for PLA2R/PLA2R1 is at 169 kDa.
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