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Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.
Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.
Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.

Anti-PLA2R1 Antibody

AMAB90775
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPLA2R1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLA2R1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0485
  • Conjugate
    Unconjugated
  • Gene ID22925
  • Target name
    PLA2R1
  • Target description
    phospholipase A2 receptor 1
  • Target synonyms
    180 kDa secretory phospholipase A2 receptor; CLEC13C; C-type lectin domain family 13 member C; M-type receptor; phospholipase A2 receptor 1, 180kD; phospholipase A2 receptor 1, 180kDa; PLA2G1R; PLA2IR; PLA2R; PLA2-R; secretory phospholipase A2 receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13018
  • Protein Name
    Secretory phospholipase A2 receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLA2R/PLA2R1 using anti-PLA2R/PLA2R1 antibody (A04442-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2R/PLA2R1 antigen affinity purified polyclonal antibody (Catalog # A04442-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2R/PLA2R1 at approximately 169 kDa. The expected band size for PLA2R/PLA2R1 is at 169 kDa.
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