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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in goblet cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in goblet cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in goblet cells.

Anti-PLAU Antibody

HPA008719
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPLAU
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLAU
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5328
  • Target name
    PLAU
  • Target description
    plasminogen activator, urokinase
  • Target synonyms
    ATF; BDPLT5; plasminogen activator, urinary; QPD; UPA; u-PA; U-plasminogen activator; URK; urokinase-type plasminogen activator
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00749
  • Protein Name
    Urokinase-type plasminogen activator
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLAU using anti-PLAU antibody (A04352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAU antigen affinity purified polyclonal antibody (Catalog # A04352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAU at approximately 48KD. The expected band size for PLAU is at 48KD.
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