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ProductsBack/Goat anti-Plasminogen Activator / PLAU

Goat anti-Plasminogen Activator / PLAU

EB06922
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPLAU
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Plasminogen Activator / PLAU Antibody
  • Delivery Days Customer
    7
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5328
  • Target name
    PLAU
  • Target description
    plasminogen activator, urokinase
  • Target synonyms
    ATF, BDPLT5, QPD, UPA, URK, u-PA, urokinase-type plasminogen activator, U-plasminogen activator, plasminogen activator, urinary
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLAU using anti-PLAU antibody (A04352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAU antigen affinity purified polyclonal antibody (Catalog # A04352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAU at approximately 48KD. The expected band size for PLAU is at 48KD.
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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in goblet cells.
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