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Immunohistochemical staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.

Anti-PLD3 Antibody

HPA012800
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLD3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23646
  • Target name
    PLD3
  • Target description
    phospholipase D family member 3
  • Target synonyms
    AD19, HU-K4, HUK4, SCA46, 5'-3' exonuclease PLD3, (S,S)-bis(monoacylglycero)phosphate synthase PLD3, choline phosphatase 3, hindIII K4L homolog, phosphatidylcholine-hydrolyzing phospholipase D3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IV08
  • Protein Name
    5'-3' exonuclease PLD3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLD3 using anti-PLD3 antibody (A01976-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD3 antigen affinity purified polyclonal antibody (Catalog # A01976-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD3 at approximately 55 kDa. The expected band size for PLD3 is at 55 kDa.
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ReactivityHuman, Mouse, Rat
TargetPLD3
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TargetPLD3
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