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ProductsBack/PLD3 / Phospholipase D3 Antibody (HRP)

PLD3 / Phospholipase D3 Antibody (HRP)

LS-C499568
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPLD3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLD3 / Phospholipase D3 Antibody (HRP)
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID23646
  • Target name
    PLD3
  • Target description
    phospholipase D family member 3
  • Target synonyms
    AD19, HU-K4, HUK4, SCA46, 5'-3' exonuclease PLD3, (S,S)-bis(monoacylglycero)phosphate synthase PLD3, choline phosphatase 3, hindIII K4L homolog, phosphatidylcholine-hydrolyzing phospholipase D3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLD3 using anti-PLD3 antibody (A01976-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD3 antigen affinity purified polyclonal antibody (Catalog # A01976-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD3 at approximately 55 kDa. The expected band size for PLD3 is at 55 kDa.
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