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Immunohistochemical staining of human duodenum shows distinct granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows distinct granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows distinct granular cytoplasmic positivity in glandular cells.

Anti-PLD5 Antibody

HPA028389
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLD5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLD5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200150
  • Target name
    PLD5
  • Target description
    phospholipase D family member 5
  • Target synonyms
    PLDC, inactive phospholipase D5, inactive PLD 5, inactive choline phosphatase 5, inactive phosphatidylcholine-hydrolyzing phospholipase D5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N7P1
  • Protein Name
    Inactive phospholipase D5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLD5 using anti-PLD5 antibody (A14197-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neoro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD5 antigen affinity purified polyclonal antibody (Catalog # A14197-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD5 at approximately 69 kDa. The expected band size for PLD5 is at 61,51,38,54 kDa.
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