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PLD5 Antibody

PACO38554
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPLD5
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PLD5 Antibody (PACO38554)
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID200150
  • Target name
    PLD5
  • Target description
    phospholipase D family member 5
  • Target synonyms
    inactive choline phosphatase 5; inactive phosphatidylcholine-hydrolyzing phospholipase D5; inactive phospholipase D5; inactive PLD 5; PLDC
  • Host
    Rabbit
  • Protein IDQ8N7P1
  • Protein Name
    Inactive phospholipase D5
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLD5 using anti-PLD5 antibody (A14197-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neoro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD5 antigen affinity purified polyclonal antibody (Catalog # A14197-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD5 at approximately 69 kDa. The expected band size for PLD5 is at 61,51,38,54 kDa.
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