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Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.

Anti-PLEKHA5 Antibody

HPA035923
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLEKHA5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLEKHA5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54477
  • Target name
    PLEKHA5
  • Target description
    pleckstrin homology domain containing A5
  • Target synonyms
    PEPP-2, PEPP2, pleckstrin homology domain-containing family A member 5, PH domain-containing family A member 5, phosphoinositol 3-phosphate-binding protein-2, pleckstrin homology domain containing, family A member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HAU0
  • Protein Name
    Pleckstrin homology domain-containing family A member 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PEPP2/PLEKHA5 using anti-PEPP2/PLEKHA5 antibody (A09135-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEPP2/PLEKHA5 antigen affinity purified polyclonal antibody (Catalog # A09135-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEPP2/PLEKHA5 at approximately 130 kDa. The expected band size for PEPP2/PLEKHA5 is at 130 kDa.
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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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