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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Anti-PLEKHG5 Antibody

HPA049570
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLEKHG5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLEKHG5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57449
  • Target name
    PLEKHG5
  • Target description
    pleckstrin homology and RhoGEF domain containing G5
  • Target synonyms
    ARHGEF45, CMTRIC, DSMA4, GEF720, HMNR4, Syx, Tech, pleckstrin homology domain-containing family G member 5, NFkB activating protein, PH domain-containing family G member 5, guanine nucleotide exchange factor 720, novel PH domain-containing protein, pleckstrin homology domain containing, family G (with RhoGef domain) member 5, synectin-binding guanine exchange factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94827
  • Protein Name
    Pleckstrin homology domain-containing family G member 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHG5 antigen affinity purified polyclonal antibody (Catalog # A08522) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHG5 at approximately 125 kDa. The expected band size for PLEKHG5 is at 111 kDa.
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