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Anti-PLTP Antibody

144-05628
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPLTP
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PLTP Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5360
  • Target name
    PLTP
  • Target description
    phospholipid transfer protein
  • Target synonyms
    BPIFE, HDLCQ9, phospholipid transfer protein, BPI fold containing family E, lipid transfer protein II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55058
  • Protein Name
    Phospholipid transfer protein
  • Scientific Description
    PLTP Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA018212EA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of PLTP using anti-PLTP antibody (A02209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLTP antigen affinity purified polyclonal antibody (Catalog # A02209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLTP at approximately 65KD. The expected band size for PLTP is at 65KD.
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