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Phospholipid Transfer Protein (PLTP) Polyclonal Antibody

CAU35618
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPLTP
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Overview

  • Supplier
    Biomatik
  • Product Name
    Phospholipid Transfer Protein (PLTP) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5360
  • Target name
    PLTP
  • Target description
    phospholipid transfer protein
  • Target synonyms
    BPIFE, HDLCQ9, phospholipid transfer protein, BPI fold containing family E, lipid transfer protein II
  • Host
    Rabbit
  • Protein IDP55058
  • Protein Name
    Phospholipid transfer protein
  • Scientific Description
    The Phospholipid Transfer Protein (PLTP) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLTP using anti-PLTP antibody (A02209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLTP antigen affinity purified polyclonal antibody (Catalog # A02209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLTP at approximately 65KD. The expected band size for PLTP is at 65KD.
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