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Figure 1. Western blot analysis of PML using anti-PML antibody (PB10084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human K562 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML antigen affinity purified polyclonal antibody (Catalog # PB10084) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML at approximately 90-160 kDa. The expected band size for PML is at 98 kDa.
Figure 1. Western blot analysis of PML using anti-PML antibody (PB10084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human K562 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML antigen affinity purified polyclonal antibody (Catalog # PB10084) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML at approximately 90-160 kDa. The expected band size for PML is at 98 kDa.
Figure 1. Western blot analysis of PML using anti-PML antibody (PB10084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human K562 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML antigen affinity purified polyclonal antibody (Catalog # PB10084) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML at approximately 90-160 kDa. The expected band size for PML is at 98 kDa.

Anti-PML Protein Antibody Picoband(r)

Research Use Only
PB10084-DYLIGHT594
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPML
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PML Protein Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID5371
  • Target name
    PML
  • Target description
    PML nuclear body scaffold
  • Target synonyms
    E3 SUMO-protein ligase PML; MYL; PML/RARA fusion; PP8675; probable transcription factor PML; promyelocytic leukemia protein; promyelocytic leukemia, inducer of; protein PML; RING finger protein 71; RING-type E3 SUMO transferase PML; RNF71; TRIM19; tripartite motif protein TRIM19; tripartite motif-containing protein 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29590
  • Protein Name
    Protein PML
  • Scientific Description
    Boster Bio Anti-PML Protein Antibody Picoband® catalog # PB10084. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203