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Anti-PNPLA2 Antibody

144-06245
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetPNPLA2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PNPLA2 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57104
  • Target name
    PNPLA2
  • Target description
    patatin like phospholipase domain containing 2
  • Target synonyms
    1110001C14Rik; adipose triglyceride lipase; ATGL; calcium-independent phospholipase A2; desnutrin; FP17548; iPLA2zeta; IPLA2-zeta; mutant patatin-like phospholipase domain containing 2; patatin-like phospholipase domain-containing protein 2; PEDF-R; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2; TTS-2.2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AD5
  • Protein Name
    Patatin-like phospholipase domain-containing protein 2
  • Scientific Description
    PNPLA2 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATGL/PNPLA2 using anti-ATGL/PNPLA2 antibody (M01800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATGL/PNPLA2 antigen affinity purified monoclonal antibody (Catalog # M01800) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATGL/PNPLA2 at approximately 55 kDa. The expected band size for ATGL/PNPLA2 is at 55 kDa.
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