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Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.

Anti-PNPLA2 Antibody

HPA075175
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPNPLA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PNPLA2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57104
  • Target name
    PNPLA2
  • Target description
    patatin like domain 2, triacylglycerol lipase
  • Target synonyms
    1110001C14Rik, ATGL, FP17548, PEDF-R, TTS-2.2, TTS2, iPLA2zeta, patatin-like phospholipase domain-containing protein 2, IPLA2-zeta, TTS2.2, adipose triglyceride lipase, calcium-independent phospholipase A2, calcium-independent phospholipase A2-zeta, desnutrin, mutant patatin-like phospholipase domain containing 2, patatin like phospholipase domain containing 2, pigment epithelium-derived factor, pigment epithelium-derived factor receptor, transport-secretion protein 2.2, triglyceride hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AD5
  • Protein Name
    Patatin-like phospholipase domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ATGL/PNPLA2 using anti-ATGL/PNPLA2 antibody (M01800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATGL/PNPLA2 antigen affinity purified monoclonal antibody (Catalog # M01800) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATGL/PNPLA2 at approximately 55 kDa. The expected band size for ATGL/PNPLA2 is at 55 kDa.
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