Anti-Podoplanin (MAP tag) [PMab-1]

Absolute Antibody

Anti-Podoplanin (MAP tag) [PMab-1]

7

This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), wh

This antibody binds to the PLAG domain of murine PDPN and is, therefore, able to block its interaction with C-type lectin-like receptor-2 (CLEC-2) (Yamada, 2017). This has a number of potential therapeutic applications, including the suppression of lymphangiogenesis and inflammatory macrophage infiltration at the site of wound healing in mouse corneal suture and ear section models (Maruyama et al, 2014), as well as suppression of transplant rejection. This antibody possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), and can, therefore, be used to detect and purify tagged proteins in a range of assay formats (Fujii et al, 2016). PMab-1 performs well in immunohistochemical analysis, and has been used to stain the mouse melanoma B16-F10 and mouse podoplanin-expressing CHO transfectants (Kaji et al, 2012). The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012). The specificity of this antibody has been confirmed in Western blot analysis (Kaji et al, 2012), while Western blot analysis has also revealed that activation of LPS-induced inflammatory pathways, including the MAPK and NF-kappaB pathways, is affected by PMab-1 (Maruyama et al, 2014). The mouse-rat chimeric antibody mPMab-1 is derived from PMab-1 (Yamada et al, 2017).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

ELISA; WB; IHC; Block; epitope tag

Research Use Only

Monoclonal

PMab-1

Pdpn

podoplanin

aggrus; E11; glycoprotein 38; Gp3; Gp38; OTS; OTS-8; PA2.; PA2.26 antigen; podoplanin; RAND; RANDAM-2; T1; T1a; T1alpha; T1-alpha; transmembrane glycoprotein E11

Mouse

IgG1

Podoplanin

This chimeric mouse antibody was made using the variable domain sequences of the original rat format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.

-20°C,2°C to 8°C

12352203