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Formalin-fixed and paraffin embedded rat colitis tissue labeled with Rabbit Anti-Podoplanin/gp36 Polyclonal Antibody (bs-1048R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colitis tissue labeled with Rabbit Anti-Podoplanin/gp36 Polyclonal Antibody (bs-1048R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colitis tissue labeled with Rabbit Anti-Podoplanin/gp36 Polyclonal Antibody (bs-1048R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Podoplanin Polyclonal Antibody

BS-1048R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPdpn
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Podoplanin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID14726
  • Target name
    Pdpn
  • Target description
    podoplanin
  • Target synonyms
    aggrus; E11; glycoprotein 38; Gp3; Gp38; OTS; OTS-8; PA2.; PA2.26 antigen; podoplanin; RAND; RANDAM-2; T1; T1a; T1alpha; T1-alpha; transmembrane glycoprotein E11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ62011
  • Protein Name
    Podoplanin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Can we enhance osteoporotic metaphyseal fracture healing through enhancing ultrastructural and functional changes of osteocytes in cortical bone with low-magnitude high-frequency vibration? Choy MV et al., 2020 Mar, FASEB J
    Read more
  • Phospholipase Cepsilon plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells. Umezawa K et al., 2019 Jan 11, Respir Res
    Read more
  • A practical and sensitive method of quantitating lymphangiogenesis in vivo. Majumder M et al., 2013 Jul, Lab Invest
    Read more

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Figure 1. Western blot analysis of Podoplanin/gp36/Pdpn using anti-Podoplanin/gp36/Pdpn antibody (A01124-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Podoplanin/gp36/Pdpn antigen affinity purified polyclonal antibody (Catalog # A01124-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Podoplanin/gp36/Pdpn at approximately 37KD. The expected band size for Podoplanin/gp36/Pdpn is at 37KD.
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