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Anti-POLR1C Antibody

144-00269
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPOLR1C
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-POLR1C Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9533
  • Target name
    POLR1C
  • Target description
    RNA polymerase I and III subunit C
  • Target synonyms
    AC40; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide; DNA-directed RNA polymerases I and III subunit RPAC1; HLD11; polymerase (RNA) I polypeptide C, 30kDa; polymerase (RNA) I subunit C; RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA39; RPA40; RPA5; RPAC1; RPC40; TCS3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15160
  • Protein Name
    DNA-directed RNA polymerases I and III subunit RPAC1
  • Scientific Description
    POLR1C Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR1C using anti-POLR1C antibody (A07025-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1C antigen affinity purified polyclonal antibody (Catalog # A07025-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1C at approximately 40 kDa. The expected band size for POLR1C is at 40 kDa.
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