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Western Blot Positive WB detected in: HEK293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue, Mouse kidney tissue, Rat heart tissue All lanes: POLR1C antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 39 kDa Observed band size: 40 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue, Mouse kidney tissue, Rat heart tissue All lanes: POLR1C antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 39 kDa Observed band size: 40 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue, Mouse kidney tissue, Rat heart tissue All lanes: POLR1C antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 39 kDa Observed band size: 40 kDa

POLR1C Antibody

CSB-PA018324LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOLR1C
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLR1C Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9533
  • Target name
    POLR1C
  • Target description
    RNA polymerase I and III subunit C
  • Target synonyms
    AC40, HLD11, RPA39, RPA40, RPA5, RPAC1, RPC40, TCS3, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit C, DNA-directed RNA polymerases I and III 40 kDa polypeptide, RNA polymerase I subunit C, RNA polymerases I and III subunit AC1, polymerase (RNA) I polypeptide C, 30kDa, polymerase (RNA) I subunit C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15160
  • Protein Name
    DNA-directed RNA polymerases I and III subunit RPAC1
  • Scientific Description
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLR1C using anti-POLR1C antibody (A07025-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1C antigen affinity purified polyclonal antibody (Catalog # A07025-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1C at approximately 40 kDa. The expected band size for POLR1C is at 40 kDa.
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