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Anti-POLR2E Antibody

144-01755
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPOLR2E
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-POLR2E Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5434
  • Target name
    POLR2E
  • Target description
    RNA polymerase II, I and III subunit E
  • Target synonyms
    RPABC1, RPB5, XAP4, hRPB25, hsRPB5, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerase subunit RPABC1, RNA polymerase II subunit E, RNA polymerases I, II, and III subunit ABC1, RPB5 homolog, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, polymerase (RNA) II subunit E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19388
  • Protein Name
    DNA-directed RNA polymerases I, II, and III subunit RPABC1
  • Scientific Description
    POLR2E Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (A08765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPB5/POLR2E antigen affinity purified polyclonal antibody (Catalog # A08765-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPB5/POLR2E at approximately 23 kDa. The expected band size for RPB5/POLR2E is at 23-25 kDa.
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