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Immunohistochemical staining of human kidney shows distinct nucleolar positivity in tubular cells.
Immunohistochemical staining of human kidney shows distinct nucleolar positivity in tubular cells.
Immunohistochemical staining of human kidney shows distinct nucleolar positivity in tubular cells.

Anti-POMP Antibody

HPA026995
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOMP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POMP Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51371
  • Target name
    POMP
  • Target description
    proteasome maturation protein
  • Target synonyms
    C13orf12, HSPC014, PNAS-110, PRAAS2, UMP1, proteasome maturation protein, 2510048O06Rik, hUMP1, proteassemblin, protein UMP1 homolog, voltage-gated K channel beta subunit 4.1, voltage-gated potassium channel beta subunit 4.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y244
  • Protein Name
    Proteasome maturation protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of POMP using anti-POMP antibody (A04837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMP antigen affinity purified polyclonal antibody (Catalog # A04837-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMP at approximately 16 kDa. The expected band size for POMP is at 16 kDa.
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