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WB analysis of various sample lysates using GTX66553 POMP antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66553 POMP antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66553 POMP antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:3000 Loading : 25microg per lane

POMP antibody

GTX66553
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPOMP
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Overview

  • Supplier
    GeneTex
  • Product Name
    POMP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51371
  • Target name
    POMP
  • Target description
    proteasome maturation protein
  • Target synonyms
    2510048O06Rik; C13orf12; HSPC014; hUMP1; PNAS-110; PRAAS2; proteasome maturation protein; proteassemblin; protein UMP1 homolog; UMP1; voltage-gated K channel beta subunit 4.1; voltage-gated potassium channel beta subunit 4.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y244
  • Protein Name
    Proteasome maturation protein
  • Scientific Description
    The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5 UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.[provided by RefSeq, Aug 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POMP using anti-POMP antibody (A04837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMP antigen affinity purified polyclonal antibody (Catalog # A04837-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMP at approximately 16 kDa. The expected band size for POMP is at 16 kDa.
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