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Anti-POMT1 Antibody

144-10281
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetPOMT1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-POMT1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10585
  • Target name
    POMT1
  • Target description
    protein O-mannosyltransferase 1
  • Target synonyms
    LGMD2K, LGMDR11, MDDGA1, MDDGB1, MDDGC1, RT, protein O-mannosyl-transferase 1, dolichyl-phosphate-mannose--protein mannosyltransferase 1, testis tissue sperm-binding protein Li 57p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6A1
  • Protein Name
    Protein O-mannosyl-transferase 1
  • Scientific Description
    POMT1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POMT1 using anti-POMT1 antibody (A04295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMT1 antigen affinity purified polyclonal antibody (A04295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMT1 at approximately 75 kDa. The expected band size for POMT1 is at 85 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA125511(POMT1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
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