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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA125511(POMT1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA125511(POMT1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA125511(POMT1 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

POMT1 Antibody

CSB-PA125511
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOMT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    POMT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10585
  • Target name
    POMT1
  • Target description
    protein O-mannosyltransferase 1
  • Target synonyms
    LGMD2K, LGMDR11, MDDGA1, MDDGB1, MDDGC1, RT, protein O-mannosyl-transferase 1, dolichyl-phosphate-mannose--protein mannosyltransferase 1, testis tissue sperm-binding protein Li 57p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6A1
  • Protein Name
    Protein O-mannosyl-transferase 1
  • Scientific Description
    The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POMT1 using anti-POMT1 antibody (A04295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMT1 antigen affinity purified polyclonal antibody (A04295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMT1 at approximately 75 kDa. The expected band size for POMT1 is at 85 kDa.
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