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Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.

Anti-POMT2 Antibody

HPA003663
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOMT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POMT2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29954
  • Target name
    POMT2
  • Target description
    protein O-mannosyltransferase 2
  • Target synonyms
    LGMD2N, LGMDR14, MDDGA2, MDDGB2, MDDGC2, protein O-mannosyl-transferase 2, dolichyl-phosphate-mannose--protein mannosyltransferase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKY4
  • Protein Name
    Protein O-mannosyl-transferase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of POMT2 using anti-POMT2 antibody (A04876-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMT2 antigen affinity purified polyclonal antibody (Catalog # A04876-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMT2 at approximately 84KD. The expected band size for POMT2 is at 84KD.
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